Steroid hormones biochemistry

Acne is often present. Acne conglobata is a particularly severe form of acne that can develop during steroid abuse or even after the drug has been discontinued. Infections are a common side effect of steroid abuse because of needle sharing and unsanitary techniques used when injecting the drugs into the skin. These are similar risks to IV drug abusers with increased potential to acquire blood-borne infections such as hepatitis and HIV/AIDS . Skin abscesses may occur at injection sites and may spread to other organs of the body. Endocarditis or an infection of the heart valves is not uncommon.

The secretion of cortisol is mainly controlled by three inter-communicating regions of the body, the hypothalamus in the brain, the pituitary gland and the adrenal gland . This is called the hypothalamic–pituitary–adrenal axis. When cortisol levels in the blood are low, a group of cells in a region of the brain called the hypothalamus releases corticotrophin-releasing hormone , which causes the pituitary gland to secrete another hormone, adrenocorticotropic hormone , into the bloodstream. High levels of adrenocorticotropic hormone are detected in the adrenal glands and stimulate the secretion of cortisol, causing blood levels of cortisol to rise. As the cortisol levels rise, they start to block the release of corticotrophin-releasing hormone from the hypothalamus and adrenocorticotropic hormone from the pituitary. As a result the adrenocorticotropic hormone levels start to drop, which then leads to a drop in cortisol levels. This is called a negative feedback loop.

Neural injections of Bromodeoxyuridine (BrdU) were applied to males of both groups to test for neurogenesis . Analysis showed that testosterone and dihydrotestosterone regulated adult hippocampal neurogenesis (AHN). Adult hippocampal neurogenesis was regulated through the androgen receptor in the wild-type male rats, but not in the TMF male rats. To further test the role of activated androgen receptors on AHN, flutamide , an antiandrogen drug that competes with testosterone and dihydrotestosterone for androgen receptors , and dihydrotestosterone were administered to normal male rats. Dihydrotestosterone increased the number of BrdU cells, while flutamide inhibited these cells.

An analytical method based on on-line SPE-LC-APCI-MS/MS has been developed for the detection and quantification of eight selected estrogenic and progestagenic steroid hormones; estrone (E1), 17β-estradiol (E2), estriol (E3), 17α-ethinylestradiol (EE2), levonorgestrel (LEVO), medroxyprogesterone (MEDRO), norethindrone (NORE) and progesterone (PROG) in wastewater matrices. The injection volume could range from 1 to 10-mL according to the expected concentration of steroid hormones in matrix. The method characteristics are: analysis time per sample (<15 min), acceptable recovery values (71-95%), good precision (RSD ≤ 10%) and limits of detection at the low-nanogram per liter levels in affluent and effluent wastewaters (8-60 ng L(-1)). In particular, a detailed discussion of optimization parameters impacting overall performance of the method has been presented (sample collection, filtration and storage). All optimization and validation experiments for the on-line SPE method and chromatographic separation were performed in environmentally-relevant wastewater matrices. This method represents a compromise between analysis time, higher sample throughput capabilities, sample volume and simplicity for the analysis of both progestagenic and estrogenic steroid hormones in a single run, with LODs and LOQs sufficiently low to detect and quantify them in environmental wastewater matrices. Thus, the applicability of the method was tested on affluent and effluent wastewaters from two wastewater treatment facilities using different processes (biological and physico-chemical) to evaluate their removal efficiency for the detected steroid hormones.

Steroid hormones biochemistry

steroid hormones biochemistry

An analytical method based on on-line SPE-LC-APCI-MS/MS has been developed for the detection and quantification of eight selected estrogenic and progestagenic steroid hormones; estrone (E1), 17β-estradiol (E2), estriol (E3), 17α-ethinylestradiol (EE2), levonorgestrel (LEVO), medroxyprogesterone (MEDRO), norethindrone (NORE) and progesterone (PROG) in wastewater matrices. The injection volume could range from 1 to 10-mL according to the expected concentration of steroid hormones in matrix. The method characteristics are: analysis time per sample (<15 min), acceptable recovery values (71-95%), good precision (RSD ≤ 10%) and limits of detection at the low-nanogram per liter levels in affluent and effluent wastewaters (8-60 ng L(-1)). In particular, a detailed discussion of optimization parameters impacting overall performance of the method has been presented (sample collection, filtration and storage). All optimization and validation experiments for the on-line SPE method and chromatographic separation were performed in environmentally-relevant wastewater matrices. This method represents a compromise between analysis time, higher sample throughput capabilities, sample volume and simplicity for the analysis of both progestagenic and estrogenic steroid hormones in a single run, with LODs and LOQs sufficiently low to detect and quantify them in environmental wastewater matrices. Thus, the applicability of the method was tested on affluent and effluent wastewaters from two wastewater treatment facilities using different processes (biological and physico-chemical) to evaluate their removal efficiency for the detected steroid hormones.

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